20 X sterile RNAse free stock solution
Dilute 50 ml MOPS-SDS 20X into 950 ml of deionised water to make 1 litre of MOPS-SDS Running Buffer. Final concentration is 50mM MOPS, 50mM TRIS base, 3.47mM SDS and 1mM EDTA.
Store at room temperature
Keep away from light
R: 36/37/38
S: 26,36
MOPS SDS Running Buffer is recommended for separating medium- to large-sized proteins and is suitable for protein gel electrophoresis. The use of MOPS Buffer allows proteins to run slower than when using MES buffer.
Formedium recommend diluting 50 ml MOPS-SDS 20X into 950 ml of deionised water to make 1 litre of MOPS-SDS Running Buffer. This makes a final concentration of 50mM MOPS, 50mM TRIS base, 3.47mM SDS and 1mM EDTA. This buffer is used in 1D Gel Electrophoresis, Protein Gel Electrophoresis, Proteins, Expression, Isolation and Analysis. MOPS 1 M 209.3 g/L TRIS 1 M 121.1 g/L SDS 69.3 mM 20 g/L EDTA Na2 20.5 mM 7.6 g/L
Specifications
|
|
|
|
|
| MOPS |
1 |
M |
209.3 |
g/L |
| TRIS |
1 |
M |
121.1 |
g/L |
| SDS |
69.3 |
mM |
20 |
g/L |
| EDTA Na2 |
20.5 |
mM |
7.6 |
g/L |
Store at room temperature
Keep away from light