There are a few reasons a custom formulation might not perform as intended – and it's not always down to the formula itself.
One common issue is overcooking during sterilisation. If the media is exposed to high temperatures for too long (for example, in an autoclave), sensitive components can degrade, which may affect performance. Some ingredients – like sugars, vitamins, or specific additives – are prone to breakdown or caramelisation under excessive heat.
Other potential factors include:
- Incorrect rehydration ratios: Using too much or too little water can alter pH and nutrient availability.
- Incompatible labware or preparation methods: Reactions with containers or using non-sterile tools can introduce variability.
- Storage and shelf life: Media should be stored as instructed to maintain stability.
If you run into problems, our team can help you identify the root cause and get your research back on track.