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FM Minimal Medium

FM Minimal Medium

SKUPriceSizeQuantityAdd to Cart
FMM0103$1,459.766 x 1kg

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This media is based on well defined formulations of mineral salts, vitamins and amino acids.

Two commonly used synthetic defined minimal Dictystelium Discoideum media are FM and SIH.

FM medium developed by Franke and Kessin is used for transformation of Dictyostelium Discoideum and genomic studies. This formulation supports the growth of most strains that are capable of growing on HL5.

SIH medium is a newly developed modification of FM with regards to its amino acid compostion.  Aspartic acid is added.  Tryptophane and Lysine concentrations are significantly increased, resulting in an increase of cell density up to 5 x 10^7.

Both FM and SIH media are based on mineral salts in micro and macro concentrations supplemented with several vitamins.  Besides these components, there is a large group of amino acids present.  To facilitate genetic studies Formedium™offers complete formulations of FM and SIH as well as both media lacking various amino acids such as without Arginine, Glutamic Acid, Lysine and Methionine.


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Amino Acids  
Arg 700
Asp 300
Cys 200
GluA 500
Gly 900
His 300
Ile 600
Leu 900
Lys 900
Met 300
Phe 500
Pro 800
Thr 500
Trp 200
Val 700
Biotin 0.02
Cyanocobalamin 0.01
Folic Acid 0.2
Lipoic Acid 0.4
Riboflavin 0.5
Thiamine 0.6
Micro Elements  
Na2EDTA.2H2O 4.84
ZnSO4 2.3
H3BO3 1.11
MnCl2.4H2O 0.51
CoCl2.6H2O 0.17
CuSO4.5H2O 0.15
(NH4)6Mo7O24.4H2O 0.1
NH4Cl 53.5
CaCl2.2H2O 2.94
FeCl3 16.2
MgCl2.6H2O 81.32
KH2PO4 870
Carbon Source  
Glucose 10000
Total 19334.87

Suspend 19.3 gram powdered medium in 1 litre distilled water
Store dry at room temperature.

Requesting a Quote

It’s now even easier to build and obtain a quote prior to placing an order by converting your basket to a quote. Simply add the products to your shopping basket as if placing an order.

Once all required products have been added to the basket, proceed to the checkout where you should complete the billing and delivery details.  Check the Research declaration and the terms & conditions boxes and click the ‘Request Quote’.

Accepting a Quote

To accept your quote locate the original email with the quote attached and click the "Accept quote and proceed to payment" link.

You will be redirected to our website where you will need to login with your username and password (unless already logged in).

If you do not have an online account please click here to create one, or if you are unsure of your password, click here to reset it.

Once you have logged in, a summary of your order will appear, simply scroll down to enter your payment details or purchase order number and click "Place Order".

Orders within the UK

Orders within the UK with an order value of £300 or more (excluding VAT) will be delivered free of charge. 
Orders within the UK with an order value of less than £300 (excluding VAT) will be charged delivery of £13.50. 

Orders from the European Union

The cost of shipping orders will vary by destination and weight. 
All freight and transportation charges will be quoted in advance and are clearly indicated on the invoice. 

Orders from the US

Deliveries to the US start from less than $32. Formedium can assist you with or organise your US import permits. 
All freight charges are based on weight of order. Import tax is the responsibility of the purchaser and their clearing agents. 

Orders from the rest of the world

Formedium deliver worldwide. Customs restrictions may apply so please do contact us if you have any queries. 
The cost of shipping orders will vary by destination and weight. 
All freight and transportation charges will be quoted in advance and are clearly indicated on the invoice.  Import tax is the responsibility of the purchaser and their clearing agents.

Being a producer of powdered media, Formedium Ltd has the knowledge, equipment and laboratories to produce custom made media according to the end users specifications.  If you are interested in the production of a custom made media, please complete the form below or alternatively contact us on 00 44 (0)1485 609069 or

We are pleased to offer unpublished discounts on large volume purchases, please email us at with your enquiry or complete the enquiry form below.

To review our research publications library where Formedium products have been used, please view the research publications page.

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Name & Registered Office: Formedium Ltd. Norfolk House, 22-24 Market Place, Swaffham, Norfolk, England, PE37 7QH Registered in England and Wales. Company No. 04351225. VAT No. GB 803727440

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