Knowledge
Neidhardt Mops Minimal Medium and Drop-Outs
Neidhardt FC, Bloch PL, Smith DF. J Bacteriol. 1974 Sep; 119(3) : 736-47.
Neidhardt MOPS minimal medium supports growth of Escherichia coli, Salmonella typhimurium and other enterobacteria at rates and yields comparable to traditional media such as complex LB media and defined M9 medium.
The medium supplied by Formedium is a slight variant on that defined by Neidhardt et al. Modifications were made by Blattner et al.
Neidhardt MOPS minimal medium is a rich defined medium which should allow repeatable high growth rates.
Concentration of all constituents is defined and can be increased independently.
Custom made productions of Neidhardt MOPS minimal medium with altered concentrations of certain constituents can be produced upon request.
Composition of Neidhardt MOPS minimal medium
- All levels of micronutrients (eg. copper, manganese etc.) are set to a level that is saturating for growth but also adequately low that there is no possibility of growth inhibition.
- Macro elements such as Phosphate, Sulfate, Nitrogen are present at a sufficiently low level to permit isotopic labeling.
- Both Magnesium and Calcium ions are present in a concentration suitably enough to support outer membrane stability as E. coli needs a lot of Mg2+ and Ca2+ to bridge the highly negatively charged LPS molecules in its outer membrane.
- Buffering capacity is provided by Morpholinopropane sulfonic acid MOPS, (pKa 7.2). MOPS is a member of the GOOD buffer range and provides excellent buffer capacity without any apparent inhibitory effect on growth.
- Glucose is the carbon source present in Neidhardt MOPS minimal medium. Neidhardt’s Basal salt mixture is available with two different concentration of Glucose, 2 grams/l and 20 grams/l.
- Within the formulation of Neidhardt MOPS minimal medium 20 amino acids are present. This group of amino acids provides excellent opportunities for two hybrid screenings with E. coli strains. Besides these amino acids nucleotides and vitamins are present to support a vigorous growth.
Neidhardt MOPS minimal medium is supplied as a kit of two mixtures;
- Neidhardt basal salt mixture; Containing all micro- and macro salt, buffers and glucose.
- Neidhardt supplement mixture; Containing all amino acids, vitamins, purines and pyrimidines.
Neidhardt basal salt mixture is used in a concentration of 32.9 grams/l and Neidhardt supplement mixture in a concentration of 3.1 grams/l. Suspend 32.9 grams of Neidhardt basal salt mixture plus 3.1 grams of Neidhardt supplement mixture in one litre of distilled water. Sterilisation by means of filtration. After dissolving a slight precipitation might occur. It is not clear what this precipitate might be. Removing it from the medium solution by filtration has showed no negative effect on cell growth.
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Michael Hannan
University of Dundee
“Formedium's technical support assisted us to make ready-made Aspergillus medium, saving us countless hours in preparation and have consistency across experiments going forwards. Their cost-effectiveness impressed us so much that we've now switched all our medium consumables to them!”
Norman Van Rhijn
University of Manchester
“Formedium has been a long-term supplier of media products to our fermentation facility. From day one, their product quality and customer service has been second to none. Impressively, their outstanding service has always been delivered alongside competitive pricing and a sense of that personal touch. Formedium have even formulated and packaged media, specifically for our needs, and their technical support has always been on hand when needed.”
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Cambridge Biomedical Campus
