Knowledge
Bacteriophage Lambda Medium Formulations
Formedium™ manufactures a large range of media for maintenance and propagation of bacteriophage lambda in Escherichia Coli cells as described by Blattner, F. et al., Science, 196, 161, 1977.
NZCYM medium, as originally developed by Blattner, is available in several modification providing the researcher to select the optimum medium formulation for a specific strain.
Tryptone or Casein, Yeast Extract and Casamino acids are sources of nitrogen within this group of media. Peptides and amino acids are abundantly present in Tryptone. Yeast Extract is an abundant source of amino acids, vitamins, nucleotides and carbohydrates.
Due to its high degree of digestion, Casamino acids is a rich source of free amino acids.
Due to its high degree of digestion, Casamino acids is a rich source of free amino acids.
Sodium ions for transport and a suitable osmotic balance are provided by Sodium Chloride.
Magnesium Sulfate provides magnesium ions required in a variety of enzymatic reactions, including DNA replication.
To optimise binding of phage lambda to Escherichia Coli cells add 0.2% Maltose to the medium for inducing lambda receptors (LampB) on host cells.
Blattner, F.R., et al., Charon phages: Safer derivatives of bateriophage for DNA cloning, Science, 196, 161, (1977). Difco manual 11th ed., Sparks, MD (1998), 22-23. Sambrook, J,., E. F. Fritsch, and T. Maniatis, 1989, Molecular cloning: a laboratory manual, 2nd edition ed., Cold Spring Harbour laboratory, Cold Spring Harbour, N.Y. Assubel, F.M., R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith and K. Struhl, Current protocols in molecular biology, vol. 1, Current Protocols, New York, (1994).
Why choose Formedium?
Bespoke culture
solutions
solutions
Whether you’re using industry standard media or producing your own formulation, our technical team works closely with you to deliver a consistent, reliable and cost-effective solution.
Fast, reliable
fulfilment
fulfilment
With UK-based production, global distribution and streamlined processes, we’re able to respond quickly to urgent timelines without compromising on quality.
Certified
Quality
Quality
We hold ISO 9001 certification and have ISO 14001 environmental accreditation. Every batch of powdered media and every ingredient is fully traceable.
Environmental
responsibility
We take sustainability seriously – from solar panels and rainwater collection at our facility to recyclable packaging and responsible procurement.
Testimonials
What our customers say
"Formedium provide not only excellent products but also a level of personal service that truly sets them apart. They take the time to understand their customers’ needs and consistently go above and beyond, all while maintaining a highly professional approach. I look forward to continuing to work with Formedium and the team for many years to come."
Michael Hannan
University of Dundee
“Formedium's technical support assisted us to make ready-made Aspergillus medium, saving us countless hours in preparation and have consistency across experiments going forwards. Their cost-effectiveness impressed us so much that we've now switched all our medium consumables to them!”
Norman Van Rhijn
University of Manchester
“Formedium has been a long-term supplier of media products to our fermentation facility. From day one, their product quality and customer service has been second to none. Impressively, their outstanding service has always been delivered alongside competitive pricing and a sense of that personal touch. Formedium have even formulated and packaged media, specifically for our needs, and their technical support has always been on hand when needed.”
Dr Shane Palmer
Cambridge Biomedical Campus
