Knowledge
SIH Defined Minimal Media
Dictyostelium Discoideum
SIH Defined Minimal Media.
Synthetic Defined Minimal Media
Synthetic Defined Minimal Media
This media is based on well defined formulations of mineral salts, vitamins and amino acids.
Two commonly used synthetic defined minimal Dictyostelium Discoideum media are FM Media and SIH Media.
FM medium developed by Franke and Kessin is used for transformation of Dictyostelium Discoideum and genomic studies.
This formulation supports the growth of most strains that are capable of growing on HL5.
This formulation supports the growth of most strains that are capable of growing on HL5.
SIH medium is a newly developed modification of FM with regards to its amino acid compostion. Aspartic acid is added. Tryptophane and Lysine concentrations are significantly increased, resulting in an increase of cell density up to 5 x 10^7.
Both FM and SIH media are based on mineral salts in micro and macro concentrations supplemented with several vitamins. Besides these components, there is a large group of amino acids present. To facilitate genetic studies Formedium™offers complete formulations of FM and SIH as well as both media lacking various amino acids such as without Arginine, Glutamic Acid, Lysine and Methionine.
New to our range of synthetic media are FM and SIH without all amino acids. These two newly developed media, combined with a range of amino acid drop-out mixtures gives the option to make every synthetic drop-out medium required for genetic studies.
Improvement of a synthetic medium for Dictyostelium discoideum, Sang-In Han, Karl Friehs and Erwin Flaschel, Process Biochemistry, 39 (8), 925 - 930, 2004
Why choose Formedium?
Bespoke culture
solutions
solutions
Whether you’re using industry standard media or producing your own formulation, our technical team works closely with you to deliver a consistent, reliable and cost-effective solution.
Fast, reliable
fulfilment
fulfilment
With UK-based production, global distribution and streamlined processes, we’re able to respond quickly to urgent timelines without compromising on quality.
Certified
Quality
Quality
We hold ISO 9001 certification and have ISO 14001 environmental accreditation. Every batch of powdered media and every ingredient is fully traceable.
Environmental
responsibility
We take sustainability seriously – from solar panels and rainwater collection at our facility to recyclable packaging and responsible procurement.
Testimonials
What our customers say
"Formedium provide not only excellent products but also a level of personal service that truly sets them apart. They take the time to understand their customers’ needs and consistently go above and beyond, all while maintaining a highly professional approach. I look forward to continuing to work with Formedium and the team for many years to come."
Michael Hannan
University of Dundee
“Formedium's technical support assisted us to make ready-made Aspergillus medium, saving us countless hours in preparation and have consistency across experiments going forwards. Their cost-effectiveness impressed us so much that we've now switched all our medium consumables to them!”
Norman Van Rhijn
University of Manchester
“Formedium has been a long-term supplier of media products to our fermentation facility. From day one, their product quality and customer service has been second to none. Impressively, their outstanding service has always been delivered alongside competitive pricing and a sense of that personal touch. Formedium have even formulated and packaged media, specifically for our needs, and their technical support has always been on hand when needed.”
Dr Shane Palmer
Cambridge Biomedical Campus
