M13 Phage and ssDNA Bacteriophages Medium Formulations
YT and 2X YT are designed for cultivation of recombinant strains of Escherichia Coli . These media are also used for maintenance and propagation of M13 bacteriophage and several types of ssDNA bacteriophage.
YT is a moderately rich medium, where 2x YT is a nutritional rich medium providing nitrogen and growth factors allowing bacteriophage to reproduce in large quantities without exhausting the host cells. The presence of amino acids, nucleotides, vitamins and other nutritional elements, that the cell would otherwise have to synthesize, stimulates a luxurious growth and high yields of the host cells. Sodium Chloride is present in the medium to provide a suitable osmolarity.
Difco manual 11th ed., Sparks, MD (1998), 22-23. Sambrook, J,., E. F. Fritsch, and T. Maniatis, 1989, Molecular cloning: a laboratory manual, 2nd edition ed., Cold Spring Harbour laboratory, Cold Spring Harbour, N.Y. Assubel, F.M., R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith and K. Struhl, Current protocols in molecular biology, vol. 1, Current Protocols, New York, (1994). Davis, L.G., M.D. Dibner and J.F. Battey, Basic methods in molecular biology, Elsevier, new York, (1986).
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