Schizosaccharomyces Pombe Minimal Defined Media

EMM and Related Media Composition
Component EMM EMM w/o Dextrose EMM-Low Glucose EMMS (1,2 M Sorbitol) EMM w/o Phosphate EMM w/o Nitrogen EMM SP MB Medium MMA Agar Medium Units
Carbon Source
Dextrose 20.00 – 5.00 20.00 20.00 20.00 20.00 – – g
Glucose – – – – – – – 5.00 10.00 g
Sorbitol – – – 216.00 – – – – – g
Amino Acids
Adenine – – – – – – 50.00 – – mg
Histidine – – – – – – 50.00 – – mg
Lysine – – – – – – 50.00 – – mg
L-Leucine – – – – – – – 150.00 – mg
Uracil – – – – – – – 150.00 – mg
Minerals
Phtalic Acid K+ 3.00 3.00 3.00 3.00 3.00 3.00 3.00 – – g
Na₂HPO₄ 2.20 2.20 2.20 2.20 – 2.20 2.20 – – g
NH₄Cl 5.00 5.00 5.00 5.00 5.00 – 5.00 – – g
MgCl₂·6H₂O 1.05 1.05 1.05 1.05 1.05 1.05 1.05 – – g
CaCl₂·2H₂O 14.70 14.70 14.70 14.70 14.70 14.70 14.70 100.00 100.00 mg
KCl 1.00 1.00 1.00 1.00 1.00 1.00 1.00 – – g
Na₂SO₄ 40.00 40.00 40.00 40.00 40.00 40.00 40.00 – – mg
KH₂PO₄ – – – – – – – 500.00 1000.00 mg
K+ Acetate – – – – – – – 360.00 – mg
MgSO₄·7H₂O – – – – – – – 500.00 500.00 mg
NaCl – – – – – – – 100.00 100.00 mg
(NH₄)₂SO₄ – – – – – – – 5000.00 5000.00 mg
Vitamins
Panthotenic acid 1.00 1.00 1.00 1.00 1.00 1.00 1.00 – – mg
Nicotinic acid 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 mg
Inositol 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 mg
Biotin 1.00 1.00 1.00 1.00 1.00 1.00 1.00 0.01 0.01 mg
Ca-Pantothenate – – – – – – – 1.00 1.00 mg
Trace Elements
H₃BO₃ 0.50 0.50 0.50 0.50 0.50 0.50 0.50 0.50 0.50 mg
MnSO₄·H₂O 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 mg
ZnSO₄·7H₂O 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 mg
FeCl₃·6H₂O 0.20 0.20 0.20 0.20 0.20 0.20 0.20 0.20 0.20 mg
Na₂MoO₄·2H₂O 0.04 0.04 0.04 0.04 0.04 0.04 0.04 0.04 0.04 mg
KI 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 mg
CuSO₄·5H₂O 0.04 0.04 0.04 0.04 0.04 0.04 0.04 0.04 0.04 mg
Citric Acid 1.00 1.00 1.00 1.00 1.00 1.00 1.00 – – mg
Agar
Agar (17) (17) (17) – (17) (17) (17) – 17.00 g
Total
Total 32.30 12.30 17.30 248.30 30.10 27.30 32.50 11.90 – g/l
Total (Agar) (49.30) (23.30) (34.30) – (47.10) (44.30) (49.50) – – g/l
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