Saccharomyces Cerevisiae Synthetic Minimal Media

SD Media Composition
Component SD Agar / Glucose SD Broth / Glucose SD Agar / Glucose w/o Phosphate SD Broth / Glucose w/o Phosphate SD Agar / Galactose SD Broth / Galactose SD Agar / Raffinose SD Broth / Raffinose SD Agar / Succinate SD Broth / Succinate SD Agar / Galactose + Raffinose SD Broth / Galactose + Raffinose
Nitrogen Source g/l
Ammonium Sulphate 5 5 5 5 5 5 5 5 5 5 5 5
Carbon Source g/l
Glucose·H₂O 20 20 20 20 – – – – – – – –
Galactose – – – – 20 20 – – – – 20 20
Raffinose – – – – – – 20 20 – – 10 10
Succinate – – – – – – – – 33 33 – –
Vitamins µg/l
Biotin 2 2 2 2 2 2 2 2 2 2 2 2
Ca-Pantothenate 400 400 400 400 400 400 400 400 400 400 400 400
Folic Acid 2 2 2 2 2 2 2 2 2 2 2 2
Inositol 2000 2000 2000 2000 2000 2000 2000 2000 2000 2000 2000 2000
Nicotinic Acid 400 400 400 400 400 400 400 400 400 400 400 400
p-Aminobenzoic Acid 200 200 200 200 200 200 200 200 200 200 200 200
Pyridoxine HCl 400 400 400 400 400 400 400 400 400 400 400 400
Riboflavin 200 200 200 200 200 200 200 200 200 200 200 200
Thiamine HCl 400 400 400 400 400 400 400 400 400 400 400 400
Trace Elements µg/l
Boric Acid 500 500 500 500 500 500 500 500 500 500 500 500
Copper Sulfate 40 40 40 40 40 40 40 40 40 40 40 40
Potassium Iodide 100 100 100 100 100 100 100 100 100 100 100 100
Ferric Chloride 200 200 200 200 200 200 200 200 200 200 200 200
Manganese Sulfate 400 400 400 400 400 400 400 400 400 400 400 400
Sodium Molybdate 200 200 200 200 200 200 200 200 200 200 200 200
Zinc Sulfate 400 400 400 400 400 400 400 400 400 400 400 400
Minerals g/l
KH₂PO₄ 1 1 – – 1 1 1 1 1 1 1 1
Magnesium Sulphate anhydrous 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5
Sodium Chloride 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1
Calcium Chloride anhydrous 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1
Agar g/l
Agar 18 – 18 – 18 – 18 – 18 – 18 –
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