Product search  

or

[fusion_builder_container hundred_percent=”no” hundred_percent_height=”no” hundred_percent_height_scroll=”no” hundred_percent_height_center_content=”yes” equal_height_columns=”no” menu_anchor=”” hide_on_mobile=”small-visibility,medium-visibility,large-visibility” status=”published” publish_date=”” class=”” id=”” background_color=”” background_image=”” background_position=”center center” background_repeat=”no-repeat” fade=”no” background_parallax=”none” enable_mobile=”no” parallax_speed=”0.3″ video_mp4=”” video_webm=”” video_ogv=”” video_url=”” video_aspect_ratio=”16:9″ video_loop=”yes” video_mute=”yes” video_preview_image=”” border_color=”” border_style=”solid” margin_top=”” margin_bottom=”” padding_top=”” padding_right=”” padding_bottom=”” padding_left=”” type=”legacy”][fusion_builder_row][fusion_builder_column type=”1_1″ layout=”1_1″ spacing=”” center_content=”no” link=”” target=”_self” min_height=”” hide_on_mobile=”small-visibility,medium-visibility,large-visibility” class=”” id=”” background_color=”” background_image=”” background_image_id=”” background_position=”left top” background_repeat=”no-repeat” hover_type=”none” border_color=”” border_style=”solid” border_position=”all” padding_top=”” padding_right=”” padding_bottom=”” padding_left=”” margin_top=”” margin_bottom=”” animation_type=”” animation_direction=”left” animation_speed=”0.3″ animation_offset=”” last=”true” border_sizes_top=”0″ border_sizes_bottom=”0″ border_sizes_left=”0″ border_sizes_right=”0″ first=”true” type=”1_1″][fusion_text columns=”” column_min_width=”” column_spacing=”” rule_style=”default” rule_size=”” rule_color=”” content_alignment_medium=”” content_alignment_small=”” content_alignment=”” hide_on_mobile=”small-visibility,medium-visibility,large-visibility” sticky_display=”normal,sticky” class=”” id=”” font_size=”” fusion_font_family_text_font=”” fusion_font_variant_text_font=”” line_height=”” letter_spacing=”” text_color=”” animation_type=”” animation_direction=”left” animation_speed=”0.3″ animation_offset=””]

Animal Free Media for Growth of E. Coli Cultures

Formedium has added six animal free media to its Vegetal range of culture media for growth of E. coli. In all six Vegetal media, Tryptone has been replaced by Soya Peptone, for the following formulations of LB Miller medium, LB Lennox medium and 2YT medium. Soya Peptone and Yeast Extract are present as a general nitrogen source for growth and protein expression. Both are excellent sources for nitrogen and additionally Yeast Extract provides B-type vitamins, carbohydrates and growth factors.

In vegetal media,thus avoiding any contamination of medium with constituents of animal origin, Tryptone is replaced by Soya Peptone. In contrast to Tryptone, which is a pancreatic digest of bovine milk casein, its replacement Soya Peptone is made by Papaic hydrolysis of Soya Bean meal. Nitrogen content of Soya Peptone (9.5 %) compared to Tryptone is slightly lower and the same for its amino nitrogen content (3 %). This difference of less than two percent does not notably influence growth and protein expression.

“A comparative study between the use of Tryptone as a nitrogen source and Soya Peptone as an alternative source showed no difference in growth.”

In liquid media cell growth of E. Coli is measured by optical density at 680nm this did not show any significant variance between growth curves of cells cultured in LB Miller Broth, LB Lennox Broth and 2YT Broth medium containing Tryptone or Soya Peptone.
In solid media counts of Colony Forming Units (CFU) of LB Lennox Agar, LB Miller Agar and 2YT Agar containing Tryptone or Soya Peptone did not significantly differ from each other.

No substantial difference could be noted between media containing Soya Peptone and Tryptone. Soya Bean Peptone performed as well compared to the use of Tryptone in both liquid and solid media.

In all three media LB Miller Vegetal medium, LB Lennox Vegetal medium and 2YT Vegetal medium including Soya Peptone, no animal sourced constituents are present. Due to the absence of animal sourced components Formedium certifies;

Therefore Formedium Ltd. takes the position that this product is free of any risk in terms of Bovine Spongiform Encephalothy (BSE) or Transmissible Spongiform Encephalopathy (TSE).

Formedium Animal Free Media

Methods:
Broth culture: Cell growth was measured in LB Lennox, LB Miller and 2YT medium.
Each medium was present in a formulation containing Tryptone or Soya Peptone.

 

Formedium Animal Free Media

Methods:
Agar culture on plates: The number of Colony Forming units was measured in LB Lennox, LB Miller and 2YT Agar plates.
Each medium was present in a formulation containing Tryptone or Soya Peptone.

[/fusion_text][fusion_woo_shortcodes]

[/fusion_woo_shortcodes][/fusion_builder_column][/fusion_builder_row][/fusion_builder_container]

Product search  

or


Copyright © 2023 ForMedium™. All Rights Reserved.

Name & Registered Office: Formedium Ltd. Norfolk House, 22-24 Market Place, Swaffham, Norfolk, England, PE37 7QH
Registered in England and Wales. Company No. 04351225. VAT No. GB 803727440