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| SKU | Price | Size | Quantity | Add to Cart |
|---|---|---|---|---|
| CYN0401 | $49.24 | 100g | ||
| CYN0402 | $107.08 | 250g | ||
| CYN0405 | $217.94 | 500g | ||
| CYN0410 | $398.22 | 1000g |
Scroll down on the table to view all product variations or search using the boxes above each column
| Formula | mg/L |
| Biotin | 0.002 |
| Ca-Panthotenate | 0.4 |
| Folic acid | 0.002 |
| Inositol | 2 |
| Nicotinic Acid, (Niacin) | 0.4 |
| p-Aminobenzoic Acid | 0.2 |
| Pyridoxine HCl | 0.4 |
| Riboflavin | 0.2 |
| Thiamine HCl | 0.4 |
| Boric Acid | 0.5 |
| Copper Sulfate | 0.04 |
| Potassium Iodide | 0.1 |
| Ferric Chloride | 0.2 |
| Manganese Sulfate | 0.4 |
| Sodium Molybdate | 0.2 |
| Zinc Sulfate | 0.4 |
| Potassium Phosphate, monobasic | 1000 |
| Magnesium Sulphate. anh | 500 |
| Sodium Chloride | 100 |
| Calcium Chloride.anh | 100 |
| Ammonium Sulphate | 5000 |
Suspend 6.9 gram powdered medium in 1 litre distilled water
Store dry at room temperature
| Title | Type | Link |
|---|
Yeast Nitrogen Base without amino acids (YNB w/o AA) is used for selecting yeasts based on amino acid and carbohydrate requirements. In Yeast Nitrogen Base without amino acids, Ammonium sulphate is included as a readily available nitrogen source for nitrogen assimilation. The medium includes all other vitamins, mineral salts and trace elements required for a vigorous growth of yeast cells.
Compared to full Yeast Nitrogen Base medium, Yeast Nitrogen Base without amino acids lacks Histidine, Methionine and Trypthophan. In combination with Drop Out medium supplement mixtures, Yeast Nitrogen Base without Amino acids provides an excellent medium to cultivate and to select auxothophic strains of yeast that requires the addition of essential nutrients like amino acids and/or vitamins.
The addition of a carbon source like glucose, galactose or raffinose is required. Synthetic Dextrose Minimal medium (SD medium) and Synthetic Complete medium (SC medium) are based upon Yeast Nitrogen Base without amino acids and are complemented with glucose, galactose and raffinose as carbon sources.
Yeast, such as Saccharomyces cerevisiae, can efficiently manage nitrogen scarcity by quickly taking up and catabolising amino acids, which allows for the effective utilisation of nitrogen sources and subsequent biosynthesis of essential compounds (Kevvai et al., 2016 – see publications tab). Additionally, yeast nitrogen base without amino acids has been employed in research to induce specific growth conditions, such as filamentous growth, aiding in the investigation of gene expression patterns and cellular responses.
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Orders within the UK with an order value of £300 or more (excluding VAT) will be delivered free of charge.
Orders within the UK with an order value of less than £300 (excluding VAT) will be charged delivery of £13.50.
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Being a producer of powdered media, Formedium Ltd has the knowledge, equipment and laboratories to produce custom made media according to the end users specifications. If you are interested in the production of a custom made media, please complete the form below or alternatively contact us on 00 44 (0)1485 609069 or sales@formedium.com.
We are pleased to offer unpublished discounts on large volume purchases, please email us at info@formedium.com with your enquiry or complete the enquiry form below.
The research paper “Equipping Saccharomyces cerevisiae with an Additional Redox Cofactor Allows F420-Dependent Bioconversions in Yeast” by Misun and Marco W. Fraaije introduces the additional redox cofactor F420 into Saccharomyces cerevisiae to enable F420-dependent bioconversions in yeast (Lee, 2024). This study represents the first instance of F420 synthesis and utilisation for bioconversion in a eukaryotic organism, demonstrating the potential for developing versatile bioconversion platforms. In the research, yeast nitrogen base without amino acids was utilised as a growth medium for Saccharomyces cerevisiae.
This medium, commonly used in laboratory settings, lacks amino acids to control and manipulate the amino acid content available to the yeast, allowing researchers to study specific pathways or effects related to amino acid metabolism (Faou & Tropschug, 2004). By growing the yeast in this defined medium, the researchers could precisely regulate the nutrients available to the yeast cells, providing insights into the impact of the additional redox cofactor on the bioconversion processes within the yeast cells. The introduction of an extra redox cofactor like F420 into Saccharomyces cerevisiae creates new possibilities for bioconversion processes within yeast cells. This addition has the potential to enhance the metabolic capabilities of the yeast, enabling the utilization of F420-dependent enzymes for various redox reactions. The study contributes to expanding the understanding of redox cofactor utilization in eukaryotic organisms and highlights the potential for utilizing F420-dependent enzymes in bioconversion applications.
Equipping Saccharomyces cerevisiae with an Additional Redox Cofactor Allows F420-Dependent Bioconversions in Yeast
Authors: Misun Lee, Marco W. Fraaije
ACS Publications
Simultaneous utilization of ammonia, free amino acids and peptides during fermentative growth of Saccharomyces cerevisiae
Authors: Kaspar Kevvai, Mary-Liis Kütt, Ildar Nisamedtinov, Toomas Paalme
Wiley Online Library
A Profile of Differentially Abundant Proteins at the Yeast Cell Periphery during Pseudohyphal Growth
Authors: Tao Xu, Christian A. Shively, Rui Jin, Matthew J. Eckwahl, Craig J. Dobry, Qingxuan Song, Anuj Kumar
Journal of Biological Chemistry
To review our research publications library where Formedium products have been used, please view the research publications page.
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