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Yeast Nitrogen Base without Amino Acids and without Phosphate, supplemented with KCl

Yeast Nitrogen Base without Amino Acids and without Phosphate, supplemented with KCl

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SKUPriceSizeQuantityAdd to Cart
CYN6701$56.27100g
CYN6702$127.91250g
CYN6703$232.53500g
CYN6704$423.111kg

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The absence of phosphate (KH2PO4) provides the possibility to include alternative sources of phosphate, like P32 labeled components, or to select an optimal concentration of phosphate for certain yeast cell lines.

By omitting Potassium DiHydrogen Phosphate (KH2PO4), not only Phosphate (PO4-3) but also Potassium (K ) is omitted.

As a result yeasts are not only limited in Phosphate, but also in Potassium. This might cause problems in osmo-molarity and intercellular Potassium ion concentrations finally resulting in poor cell growth and low yields.

To avoid this problem an extra 550 mg/l (7.35 mM) of KCl is added. By adding this concentration of KCl the original concentration of K as present in YNB is restored. The extra addition of Chlorine is in almost all cases no problems to your cells.

Suspend 6.5 gram powdered medium in 1 litre distilled water
Store dry at room temperature

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Orders within the UK with an order value of £300 or more (excluding VAT) will be delivered free of charge. 
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Formedium deliver worldwide. Customs restrictions may apply so please do contact us if you have any queries. 
The cost of shipping orders will vary by destination and weight. 
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Being a producer of powdered media, Formedium Ltd has the knowledge, equipment and laboratories to produce custom made media according to the end users specifications.  If you are interested in the production of a custom made media, please complete the form below or alternatively contact us on 00 44 (0)1485 609069 or sales@formedium.com.

We are pleased to offer unpublished discounts on large volume purchases, please email us at info@formedium.com with your enquiry or complete the enquiry form below.

To review our research publications library where Formedium products have been used, please view the research publications page.

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Name & Registered Office: Formedium Ltd. Norfolk House, 24 Market Place, Swaffham, Norfolk, England, PE37 7QH Registered in England and Wales. Company No. 04351225. VAT No. GB 803727440

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