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SIH medium, developed by Hanh, Friehs and Flaschel (2004), is the next step in the development of synthetic media designed to grow D. discoideum in high cell densities.
SIH medium is an improved version of FM medium, as developed by Frank and Kessin (1977).
The main difference between SIH and FM is the novel addition of Aspartic acid (1.1 mM) and increased levels of Lysine (8.5 mM) and Tryptophane (1.7 mM). Concentrations of Cysteine, Glutamic acid, Methionine, Phenylalanine, Threonine are slightly increased as well in SIH.
The alterations in amino acid formulation of SIH medium resulted in a more even and better amino acid utilisation. Cell density of D. discoideum rose up to levels in excess of 5 x 107 cells compared to FM medium with cell density levels of 3 x 107.
Improvement of a synthetic medium for Dictyostelium discoideum,
Sang-In Han, Karl Friehs and Erwin Flaschel,
Process Biochemistry, 39 (8), 925 – 930, 2004.
Cultivation of Dictostelium discoideum on an improved synthetic medium in a conventional bioreactor.
Sang-In Han, Karl Friehs and Erwin Flaschel,
Process Biochemistry, 39, 585 – 589, 2004.
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Formula
mg/l
Amino Acids
Arg
700
Asp
300
Asp A
150
Cys
300
GluA
545
Gly
900
His
300
Ile
600
Leu
900
Lys
1250
Met
350
Phe
550
Pro
800
Thr
500
Trp
350
Val
700
Vitamins
Biotin
0.02
Cyanocobalamin
0.01
Folic Acid
0.2
Lipoic Acid
0.4
Riboflavin
0.5
Thiamine
0.6
Micro Elements
Na2EDTA.2H2O
4.84
ZnSO4
2.3
H3BO3
1.11
MnCl2.4H2O
0.51
CoCl2.6H2O
0.17
CuSO4.5H2O
0.15
(NH4)6Mo7O24.4H2O
0.1
Minerals
NH4Cl
53.5
CaCl2.2H2O
2.94
FeCl3
16.2
MgCl2.6H2O
81.32
KH2PO4
870
Carbon Source
Glucose
10000
Total
20326
Suspend 20.3 gram powdered medium in 1 litre distilled water
Store dry at room temperature.
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Being a producer of powdered media, Formedium Ltd has the knowledge, equipment and laboratories to produce custom made media according to the end users specifications. If you are interested in the production of a custom made media, please complete the form below or alternatively contact us on 00 44 (0)1485 609069 or sales@formedium.com.
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