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SIH Medium

SIH Medium

SKUPriceSizeQuantityAdd to Cart
SIH0101$81.16250g
SIH0102$270.351kg
SIH0103$1,459.766 x 1kg

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SIH medium, developed by Hanh, Friehs and Flaschel (2004), is the next step in the development of synthetic media designed to grow D. discoideum in high cell densities.

SIH medium is an improved version of FM medium, as developed by Frank and Kessin (1977).

The main difference between SIH and FM is the novel addition of Aspartic acid (1.1 mM) and increased levels of Lysine (8.5 mM) and Tryptophane (1.7 mM). Concentrations of Cysteine, Glutamic acid, Methionine, Phenylalanine, Threonine are slightly increased as well in SIH.

The alterations in amino acid formulation of SIH medium resulted in a more even and better amino acid utilisation. Cell density of D. discoideum rose up to levels in excess of 5 x 107 cells compared to FM medium with cell density levels of 3 x 107.

Improvement of a synthetic medium for Dictyostelium discoideum,
Sang-In Han, Karl Friehs and Erwin Flaschel,
Process Biochemistry, 39 (8), 925 – 930, 2004.

Cultivation of Dictostelium discoideum on an improved synthetic medium in a conventional bioreactor.
Sang-In Han, Karl Friehs and Erwin Flaschel,
Process Biochemistry, 39, 585 – 589, 2004.

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Formula
mg/l
Amino Acids  
Arg 700
Asp 300
Asp A 150
Cys 300
GluA 545
Gly 900
His 300
Ile 600
Leu 900
Lys 1250
Met 350
Phe 550
Pro 800
Thr 500
Trp 350
Val 700
Vitamins  
Biotin 0.02
Cyanocobalamin 0.01
Folic Acid 0.2
Lipoic Acid 0.4
Riboflavin 0.5
Thiamine 0.6
Micro Elements  
Na2EDTA.2H2O 4.84
ZnSO4 2.3
H3BO3 1.11
MnCl2.4H2O 0.51
CoCl2.6H2O 0.17
CuSO4.5H2O 0.15
(NH4)6Mo7O24.4H2O 0.1
Minerals  
NH4Cl 53.5
CaCl2.2H2O 2.94
FeCl3 16.2
MgCl2.6H2O 81.32
KH2PO4 870
Carbon Source  
Glucose 10000
Total 20326

Suspend 20.3 gram powdered medium in 1 litre distilled water
Store dry at room temperature.

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Orders within the UK

Orders within the UK with an order value of £250 or more (excluding VAT) will be delivered free of charge. 
Orders within the UK with an order value of less than £250 (excluding VAT) will be charged delivery of £12.50. 

Orders from the European Union

The cost of shipping orders will vary by destination and weight. 
All freight and transportation charges will be quoted in advance and are clearly indicated on the invoice. 

Orders from the US

Deliveries to the US start from less than $32. Formedium can assist you with or organise your US import permits. 
All freight charges are based on weight of order. Import tax is the responsibility of the purchaser and their clearing agents. 

Orders from the rest of the world

Formedium deliver worldwide. Customs restrictions may apply so please do contact us if you have any queries. 
The cost of shipping orders will vary by destination and weight. 
All freight and transportation charges will be quoted in advance and are clearly indicated on the invoice.  Import tax is the responsibility of the purchaser and their clearing agents.

Being a producer of powdered media, Formedium Ltd has the knowledge, equipment and laboratories to produce custom made media according to the end users specifications.  If you are interested in the production of a custom made media, please complete the form below or alternatively contact us on 00 44 (0)1485 609069 or sales@formedium.com.

We are pleased to offer unpublished discounts on large volume purchases, please email us at info@formedium.com with your enquiry or complete the enquiry form below.

To review our research publications library where Formedium products have been used, please view the research publications page.

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