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M9 Minimal Salts Base, 5x is used in preparing M9 Minimal medium used for the cultivation and maintenance of E. coli in molecular biology.
M9 is a chemical defined minimal medium supporting the growth of “wild-type” strains of E.coli. The medium can be supplemented with specific amino acids or other required nutrients allowing for selection of specific auxotrophs.
Glucose may be added as a source of carbohydrate. Nitrogen is provided by ammonium chloride final osmolarity of the medium is maintained by NaCl. Addition of magnesium and calcium increases the growth of the recombinant cells.
M9 Minimal Salts Base, 5x is a 5x concentrate and has to be diluted to a 1x concentration and subsequently supplemented with an appropriate carbon source, such as glucose. Addition of required nutritionally elements may be added.
Difco Manual, 11th edition, Sparks, MD, 272, 1998.
Davis, L.G., M.D. Dibner and J.F. Battey, Basic methods in molecular biology, Elsevier, new York, (1986).
Sambrook, J,., E. F. Fritsch, and T. Maniatis, 1989, Molecular cloning: a laboratory manual, 2nd edition ed., Cold Spring Harbour laboratory, Cold Spring Harbour, N.Y.
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Formula
g/l
Na2HPO4
33.9
KH2PO4
15
NaCl
2.5
NH4Cl
5
Final pH
6.8 ± 0.2 at 25°C
Suspend 56.4 gram powdered medium in 1 litre distilled water
Store dry at room temperature.
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