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Lee's Multi-differential Agar (LMDA) Medium

Lee’s Multi-differential Agar (LMDA) Medium

SKUPriceSizeQuantityAdd to Cart
LMDA25$3,366.0025 Kg

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Lee’s Multi-Differential Agar (LMDA) Medium is a complex nutrient medium that will detect most microorganisms commonly encountered in a brewery.

Beer is not a very appropriate medium for the development of bacteria due to its characteristics, such as the low quantity of available nutrients, the presence of alcohol, carbon dioxide and sulphur dioxide, as well as low conservation temperatures. However, growth of bacteria and wild yeast can contribute unwanted flavors to wort and beer. The degree to which it can affect flavor depends on the stage of brewing at which it occurs.


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The main types of wort and beer spoilage bacteria include: Flavobacterium, Enterobacter, Escherichia, Lactobacillus, Pediococcus, Acetobacter, Acetomonas, and Zymomonas . Wild yeast strains come in two groups: Saccharomyces and Non-Saccharomyces. Both bacteria and wild yeast can cause turbidity, haze, slime formation, over-attenuation, souring, and off flavours in the product.

LMDA Medium is used for bacteria and yeast detection / enumeration. Colonies of acid producing bacteria will be easily identified by the development of a clear zone around them because of their ability to dissolve calcium carbonate present in LMDA medium. Furthermore, colonies identification will be facilitated by the characteristic color reactions. Bromocresol green turns yellow to indicate acid production.n Both ingredients work together to differentiate between true acid producers and slight acid producers. LMDA plates can be used aerobically or anaerobically depending on which bacteria the brewer wants to detect. Incubation is done under anaerobic conditions, except when acetic acid bacteria are of major interest, then aerobic incubation is used.
An antifungal such cycloheximide can be added to suppress culture yeast growth, therefore making this media selective to bacteria.

LMDA Medium mg/l
Glucose 14,850.8
Agar 15,000.0
Bromocresol green 22.0
Calcium carbonate 5,000.0
Calcium pantothenate 2,000.0
Citric Acid 1,100.0
Dipotassium phosphate, K2HPO4 742.5
Ferrous sulfate 10.0
Magnesium sulfate anhydrous 144.3
Manganese sulfate 10.0
Monopotassium phosphate, KH2PO4 742.5
Tryptone 20,000.0
Sodium chloride 10.0
Tomato powder 9,701.7
Tween 80 500.0
Yeast extract 14,850.8


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Orders within the UK

Orders within the UK with an order value of £250 or more (excluding VAT) will be delivered free of charge.
Orders within the UK with an order value of less than £250 (excluding VAT) will be charged delivery of £12.50.

Orders from the European Union

The cost of shipping orders will vary by destination and weight.
All freight and transportation charges will be quoted in advance and are clearly indicated on the invoice.

Orders from the US

Deliveries to the US start from less than $32. Formedium can assist you with or organise your US import permits
All freight charges are based on weight of order. Import tax is the responsibility of the purchaser and their clearing agents.

Orders from the rest of the world

Formedium deliver worldwide. Customs restrictions may apply so please do contact us if you have any queries.
The cost of shipping orders will vary by destination and weight.
All freight and transportation charges will be quoted in advance and are clearly indicated on the invoice.  Import tax is the responsibility of the purchaser and their clearing agents.

Being a producer of powdered media, Formedium Ltd has the knowledge, equipment and laboratories to produce custom made media according to the end users specifications.  If you are interested in the production of a custom made media, please complete the form below or alternatively contact us on 00 44 (0)1485 609069 or

We are pleased to offer unpublished discounts on large volume purchases, please email us at with your enquiry or complete the enquiry form below.

To review research publications where Formedium products have been used, please view the publications page.

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