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L-Broth

L-Broth

SKUPriceSizeQuantityAdd to Cart
LBL0101$35.80250g
LBL0102$94.331000g
LBL0103$519.876 x 1kg

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Luria Broth Base (Miller’s Modification)

Luria Broth Base or L-Broth is based upon the Luria Broth formulation as originally developed by Miller for cultivation and maintenance of E. coli cells in molecular biology.

L-Broth is a nutritionally rich medium originally developed for growth and maintenance of recombinant E. coli strains. E. coli is grown to late log phase in LB Broth. These strains are generally derived from E. coli K12 which are deficient in B vitamin production. K12 has been ultimately modified by specific mutation into an auxothropic strain not capable of growth on nutritionally deficient medium. All nutritional requirements of E. coli strains are provided by LB Broth. Peptides and amino acids are abundantly present in Tryptone. Yeast extract is a rich source of amino acids, vitamins, nucleotides and carbohydrates. These nutritional elements in LB media, which otherwise the cell would have to synthesize, support a luxurious growth of E. coli cells.

Sodium ions for transport and osmotic balance are provided by Sodium chloride.

The concentration of NaCl in L-Broth is low compared to both LB Miller and LB Lennox formulations, respectively 10% and 5% of the NaCl concentration is present in both formulations.

These variations in Sodium chloride content make it possible to select the optimal salt concentration for a specific strain.

Miller, J.H., Experiments in molecular genetics, Cold Spring harbour Laboratory, Cold Spring harbour, New York, (1972).
Lennox, E.S., Transuction of linked genetic characters of the host by bacteripphage P1, 1, 190-206, (1955).
Sambrook, J,., E. F. Fritsch, and T. Maniatis, 1989, Molecular cloning: a laboratory manual, 2nd edition ed., Cold Spring Harbour laboratory, Cold Spring Harbour, N.Y.

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Formula
g/l
Tryptone 10
Yeast extract 5
NaCl 0.5
Final pH 7.0 ± 0.2 at 25°C

Suspend 15.5 gram powdered medium in 1 litre distilled water
Store dry at room temperature

Requesting a Quote

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Once all required products have been added to the basket, proceed to the checkout where you should complete the billing and delivery details.  Check the Research declaration and the terms & conditions boxes and click the ‘Request Quote’.

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Orders within the UK

Orders within the UK with an order value of £300 or more (excluding VAT) will be delivered free of charge. 
Orders within the UK with an order value of less than £300 (excluding VAT) will be charged delivery of £13.50. 

Orders from the European Union

The cost of shipping orders will vary by destination and weight. 
All freight and transportation charges will be quoted in advance and are clearly indicated on the invoice. 

Orders from the US

Deliveries to the US start from less than $32. Formedium can assist you with or organise your US import permits. 
All freight charges are based on weight of order. Import tax is the responsibility of the purchaser and their clearing agents. 

Orders from the rest of the world

Formedium deliver worldwide. Customs restrictions may apply so please do contact us if you have any queries. 
The cost of shipping orders will vary by destination and weight. 
All freight and transportation charges will be quoted in advance and are clearly indicated on the invoice.  Import tax is the responsibility of the purchaser and their clearing agents.

Being a producer of powdered media, Formedium Ltd has the knowledge, equipment and laboratories to produce custom made media according to the end users specifications.  If you are interested in the production of a custom made media, please complete the form below or alternatively contact us on 00 44 (0)1485 609069 or sales@formedium.com.

We are pleased to offer unpublished discounts on large volume purchases, please email us at info@formedium.com with your enquiry or complete the enquiry form below.

To review our research publications library where Formedium products have been used, please view the research publications page.

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