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FM drop-out mixture, minus Glutamic acid and w/o Lysine, 6900 mg/l

FM drop-out mixture, minus Glutamic acid and w/o Lysine, 6900 mg/l

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SKUPriceSizeQuantityAdd to Cart
FMA38$81.16100g
FMA39$162.20250g

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Synthetic Defined Minimal Media – FM Drop-out Mixture, minus Glutamic Acid and without Lysine, 6900 mg/l

This media is based on well defined formulations of mineral salts, vitamins and amino acids.

Two commonly used synthetic defined minimal Dictystelium Discoideum media are FM and SIH.

FM medium developed by Franke and Kessin is used for transformation of Dictyostelium Discoideum and genomic studies. This formulation supports the growth of most strains that are capable of growing on HL5.

SIH medium is a newly developed modification of FM with regards to its amino acid compostion.  Aspartic acid is added.  Tryptophane and Lysine concentrations are significantly increased, resulting in an increase of cell density up to 5 x 10^7.

Both FM and SIH media are based on mineral salts in micro and macro concentrations supplemented with several vitamins.  Besides these components, there is a large group of amino acids present.  To facilitate genetic studies Formedium™offers complete formulations of FM and SIH as well as both media lacking various amino acids such as without Arginine, Glutamic Acid, Lysine and Methionine.

New to our range of synthetic media are FM and SIH without all amino acids.  These two newly developed media combined with a range of amino acid drop-out mixtures gives the option to make every synthetic drop-out medium required for genetic studies.

Improvement of a synthetic medium for Dictyostelium Discoideum,
Sang-In Han, Karl Friehs and Erwin Flaschel,
Process Biochemistry, 39 (8), 925 – 930, 2004.

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Orders within the UK

Orders within the UK with an order value of £300 or more (excluding VAT) will be delivered free of charge. 
Orders within the UK with an order value of less than £300 (excluding VAT) will be charged delivery of £13.50. 

Orders from the European Union

The cost of shipping orders will vary by destination and weight. 
All freight and transportation charges will be quoted in advance and are clearly indicated on the invoice. 

Orders from the US

Deliveries to the US start from less than $32. Formedium can assist you with or organise your US import permits. 
All freight charges are based on weight of order. Import tax is the responsibility of the purchaser and their clearing agents. 

Orders from the rest of the world

Formedium deliver worldwide. Customs restrictions may apply so please do contact us if you have any queries. 
The cost of shipping orders will vary by destination and weight. 
All freight and transportation charges will be quoted in advance and are clearly indicated on the invoice.  Import tax is the responsibility of the purchaser and their clearing agents.

Being a producer of powdered media, Formedium Ltd has the knowledge, equipment and laboratories to produce custom made media according to the end users specifications.  If you are interested in the production of a custom made media, please complete the form below or alternatively contact us on 00 44 (0)1485 609069 or sales@formedium.com.

We are pleased to offer unpublished discounts on large volume purchases, please email us at info@formedium.com with your enquiry or complete the enquiry form below.

To review our research publications library where Formedium products have been used, please view the research publications page.

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