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Bacteriophage Lambda Medium Formulations

Formedium™ manufactures a large range of media for maintenance and propagation of bacteriophage lambda in Escherichia Coli cells as described by Blattner, F. et al., Science, 196, 161, 1977.

NZCYM medium, as originally developed by Blattner, is available in several modification providing the researcher to select the optimum medium formulation for a specific strain.

Tryptone or Casein, Yeast Extract and Casamino acids are sources of nitrogen within this group of media.  Peptides and amino acids are abundantly present in Tryptone. Yeast Extract is a abundantly source of amino acids, vitamins, nucleotides and carbohydrates.  Due to its high degree of digestion Casamino acids is a rich source of free amino acids.

Sodium ions for transport and a suitable osmotic balance are provided by Sodium Chloride.

Magnesium Sulfate provides magnesium ions required in a variety of enzymatic reactions, including DNA replication.

To optimise binding of phage lambda to Escherichia Coli cells add 0.2% Maltose to the medium for inducing lambda receptors (LampB) on host cells.

Blattner, F.R., et al., Charon phages: Safer derivatives of bateriophage for DNA cloning, Science, 196, 161, (1977).
Difco manual 11th ed., Sparks, MD (1998), 22-23.
Sambrook, J,., E. F. Fritsch, and T. Maniatis, 1989, Molecular cloning: a laboratory manual, 2nd edition ed., Cold Spring Harbour laboratory, Cold Spring Harbour, N.Y.
Assubel, F.M., R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith and K. Struhl, Current protocols in molecular biology, vol. 1, Current Protocols, New York, (1994).

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