Non Defined Complex Dictyostelium Discoideum

Some specific strains of Dictyostelium Discoideum are capable to grow axenically in liquid media without bacteria as food. Peptone and Yeast Extract are the main components of these media within this group. Both products are present in various ratio’s in different  Dicty media.  Peptone provides high molecular weight peptides and proteins as a nitrogen source.  Yeast Extract is a source of vitamins, co-factors and carbohydrates. Often there is an additional Phosphate buffer present to inhibit acidification of the medium during cell growth.

Commonly used HL5 and HL5-C media are also available supplemented with extra vitamins and micro-elements. This supplement  gives good results for cultures of Dicty cellines who require additional nutritional elements for starting up vigorous growth or protein synthesis.