Neidhardt FC, Bloch PL, Smith DF. J Bacteriol. 1974 Sep; 119(3) : 736-47.

Neidhardt MOPS minimal medium supports the growth of Escherichia coli, Salmonella typhimurium and other types of Enterobacteria at rates and yields comparable to traditional media such as complex LB Media and defined M9 Medium.

The Neidhardt medium supplied by Formedium is a slight variant on that defined by Neidhardt et al. Modifications were made by Blattner et al.

Neidhardt MOPS Minimal Medium is a rich defined medium which should allow repeatable high growth rates.

The concentration of all constituents is defined and can be increased independently. We can also provide custom made productions of Neidhardt MOPS Minimal Medium with altered concentrations of certain constituents. Please contact us if you would like a quote for custom made Neidhardt media.

Composition of Neidhardt MOPS Minimal Medium

• All levels of micronutrients (eg. copper, manganese etc.) are set to a level that is saturating for growth but is also adequately low enough to ensure there is no possibility of growth inhibition.
• Macro elements such as Phosphate, Sulfate, Nitrogen are present at a sufficiently low level to permit isotopic labeling.
• Both Magnesium and Calcium ions are present in the concentration suitable for the support of outer membrane stability, as E. coli needs a lot of Mg2+ and Ca2+ to bridge the highly negatively charged LPS molecules in its outer membrane.
• Buffering capacity is provided by Morpholinopropane sulfonic acid MOPS, (pKa 7.2).
• MOPS is a member of the GOOD buffer range and provides excellent buffer capacity without any apparent inhibitory effect on growth.
• In Neidhardt MOPS Minimal medium Glucose is used as carbon source. Neidhardt Basal salt mixture, used in combination with Neidhardt Supplement mixture, is supplied in a Basal Salt mixture with 2 g/l Glucose and a Basal Salt mixture containing 20 g/l Glucose.
• Neidhardt MOPS minimal is prepared by mixing both Neidhardt Basal salt mixture and Neidhardt Supplement mixture together.

Neidhardt Basal Salt mixture contains all Micro- and Macro-salts, buffers and Glucose. Neidhardt Supplement mixture contains all Amino acids, Vitamins, Purines and Pyrimidines: Neidhardt basal salt mixture; Containing all micro- and macro salt, buffers and glucose. Neidhardt supplement mixture; Containing all amino acids, vitamins, purines and pyrimidines.