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Neidhardt Drop-Out Mixtures

Neidhardt Drop-Out Mixtures

Neidhardt FC, Bloch PL, Smith DF. J Bacteriol. 1974 Sep; 119(3) : 736-47.

Neidhardt MOPS minimal medium supports growth of Escherichia coli, Salmonella typhimurium and other enterobacteria at rates and yields comparable to traditional media such as complex LB media and defined M9 medium. Neidhardt MOPS minimal medium supplied by ForMedium Ltd is a slight variant on that defined by Neidhardt et al. Modifications were made by Blattner et al.

Part the formulation of Neidhardt MOPS minimal medium are 20 amino acids, Adenine and Uracil. This group of molecules provides excellent opportunities for two hybrid screenings with E. coli strains. Depending on the system used agar plates or specific growth medium is used to grow the cells and allow selection for interaction. The most common used method is the agar plating one where cells are plated on selective medium to see of interaction takes place. Cells that have no interaction proteins should not survive on this selective medium

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