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Media for Fermentation

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LB Broth Lennox
LB Broth Miller
2xYT Broth
Terrific Broth

Illustration 1:
140L, Sterilization-In-Place, Bioreactor. Stainless steel vessels with a steam supply allow the vessel, and its contents, to be sterilized in place, without the need for an on-site autoclave. Under fully aerated conditions, where space must be reserved for gas hold-up, microbes can be cultured in batches up to 100L in this vessel.

Illustration 2:
140L Bioreactor headplate. A selection of port sizes, with some spare, ensures that the system configuration is not fixed.

Illustration 3:
Fixed and re-sterilizable addition lines for dosed control (e.g. acid and base for pH control), feeding, and inoculation.

Illustration 4:
Glass, autoclavable, vessels are the most common type of bioreactor, being simpler to operate and less demanding on utilities. Nevertheless, when connected to suitable control modules, these systems can mimic the control strategy of larger, seemingly more complex, systems.

Illustration 5:
A 15L autoclavable vessel agitating 10L of Formedium YP medium. This scale can be employed for a number of tasks including scale-up studies, process development and optimisation, medium development, and production.

Illustration text and images supplied by:
Dr Shane Palmer
Fermentation Facility
MRC Mitochondrial Biology Unit (MRC MBU)
University of Cambridge

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