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Media for Fermentation

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LB Broth Lennox
LB Broth Miller
2xYT Broth
Terrific Broth

Illustration 1:
140L, Sterilization-In-Place, Bioreactor. Stainless steel vessels with a steam supply allow the vessel, and its contents, to be sterilized in place, without the need for an on-site autoclave. Under fully aerated conditions, where space must be reserved for gas hold-up, microbes can be cultured in batches up to 100L in this vessel.

Illustration 2:
140L Bioreactor headplate. A selection of port sizes, with some spare, ensures that the system configuration is not fixed.

Illustration 3:
Fixed and re-sterilizable addition lines for dosed control (e.g. acid and base for pH control), feeding, and inoculation.

Illustration 4:
Glass, autoclavable, vessels are the most common type of bioreactor, being simpler to operate and less demanding on utilities. Nevertheless, when connected to suitable control modules, these systems can mimic the control strategy of larger, seemingly more complex, systems.

Illustration 5:
A 15L autoclavable vessel agitating 10L of Formedium YP medium. This scale can be employed for a number of tasks including scale-up studies, process development and optimisation, medium development, and production.

Illustration text and images supplied by:
Dr Shane Palmer
Fermentation Facility
MRC Mitochondrial Biology Unit (MRC MBU)
University of Cambridge

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  • Agar Granulated, Bacteriological grade is a fine granulated agar with excellent characteristics for bacteriological growth. Due to the fine granule structure of this agar dusting while handling is very low. Store dry at room temperature
  • Peptone

    $44.63$800.17
    CAS Number 73049-73-7
  • LB-Agar Lennox

    $5.73$424.48
    LB-Agar Low Salt, Lennox L Agar
  • LB-Agar Miller

    $6.63$542.65
    LB-Agar High Salt, Miller's LB Broth, Luria Agar
  • LB-Broth Lennox

    $5.28$355.08
    LB-Broth Low Salt, Lennox L Broth
    LB Broth Lennox is a nutritionally rich medium originally developed for growth and maintenance of recombinant E. coli strains. E. coli is grown to late log phase in LB Broth. These strains are generally derived from E. coli K12 which are deficient in B vitamin production. K12 has been ultimately modified by specific mutation into an auxothropic strain not capable of growth on nutritionally deficient medium. All nutritional requirements of E. coli strains are provided by LB Broth. Peptides and amino acids are abundantly present in Tryptone. Yeast extract is a rich source of amino acids, vitamins, nucleotides and carbohydrates. These nutritional elements in LB media, which otherwise the cell would have to synthesize,  support a luxurious growth of E. coli cells. Sodium ions for transport and osmotic balance are provided by Sodium chloride. The concentration of NaCl in LB Lennox is half the concentration of the LB Miller Broth formulation and ten times the concentration of LB Luria Broth formulation. These variations in Sodium chloride content make it possible to select the optimal salt concentration for a specific strain. Lennox, E.S., Transuction of linked genetic characters of the host by bacteripphage P1, 1, 190-206, (1955). Assubel, F.M., R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith and K. Struhl, Current protocols in molecular biology, vol. 1, Current Protocols, New York, (1994).
  • LB Broth Miller

    $5.28$827.66
    LB-Broth High Salt, Miller's LB Broth, Luria Broth
    LB Broth Miller is a nutritionally rich medium originally developed for growth and maintenance of recombinant E. coli strains. E. coli is grown to late log phase in LB Broth. These strains are generally derived from E. coli K12 which are deficient in B vitamin production. K12 has been ultimately modified by specific mutation into an auxothropic strain not capable of growth on nutritionally deficient medium. All nutritional requirements of E. coli strains are provided by LB Broth. Peptides and amino acids are abundantly present in Tryptone. Yeast extract is a rich source of amino acids, vitamins, nucleotides and carbohydrates. These nutritional elements in LB media, which otherwise the cell would have to synthesize,  support a luxurious growth of E. coli cells. Sodium ions for transport and osmotic balance are provided by Sodium chloride. The concentration of NaCl in LB Miller is twice the concentration of the LB Lennox Broth formulation and twenty times the concentration of LB Luria Broth formulation. These variations in Sodium chloride content make it possible to select the optimal salt concentration for a specific strain. Luria, S.E. and J.W. Burrous, Hybridization between Escherich coli and Shigella, J. Bacteriol., 74, 461-476. 1955. Luria, S.E., J.N. Adams and R.C. Ting, Transduction of lactose-utilizing ability amongst strains of E. coli and S. dysenteria and the properties of transducing phage particles, Virology, 12, 348-390, 1960. Lennox, E.S., Transuction of linked genetic characters of the host by bacteripphage P1, 1, 190-206, (1955). Sambrook, J,., E. F. Fritsch, and T. Maniatis, 1989, Molecular cloning: a laboratory manual, 2nd edition ed., Cold Spring Harbour laboratory, Cold Spring Harbour, N.Y. Assubel, F.M., R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith and K. Struhl, Current protocols in molecular biology, vol. 1, Current Protocols, New York, (1994).
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