Materials and Methods
Yeast strains, media and growth conditions
Yeast strains used in this paper are listed in Table S2. Yeast cells are grown in rich medium (YPD: 1% [wt/vol] yeast extract [ForMedium, YEM04], 2% [wt/vol] peptone [ForMedium, PEP04], 2% [wt/vol] glucose; or SMD: 0.67% yeast nitrogen base [ForMedium, CYN0410], 2% [wt/vol] glucose, 0.5% casamino acids [ForMedium, CAS03], 1× complete amino acids and vitamins supplement (see Table S3 for details), 2% glucose). Autophagy was induced by shifting mid-log phase cells from rich medium to nitrogen starvation medium (SD-N: 0.17% yeast nitrogen base without ammonium sulfate or amino acids [ForMedium,CYN0501], 2% [wt/vol] glucose) for the indicated times.
To view the full report: http://www.tandfonline.com/doi/full/10.1080/15548627.2015.1107692
